Haplotype structure strongly affects recombination in a maize genetic interval polymorphic for Helitron and retrotransposon insertions

摘要

We have asked here how the remarkable variation in maize haplotype structure affects recombination. We compared recombination across a genetic interval of 9S in 2 highly dissimilar heterozygotes that shared 1 parent. The genetic interval in the common haplotype is ≈100 kb long and contains 6 genes interspersed with gene-fragment-bearing Helitrons and retrotrans-posons that, together, comprise 70% of its length. In one hetero-zygote, most intergenic insertions are homozygous, although polymorphic, enabling us to determine whether any recombination junctions fall within them. In the other, most intergenic insertions are hemizygous and, thus, incapable of homologous recombination. Our analysis of the frequency and distribution of recombination in the interval revealed that: (ⅰ) Most junctions were circumscribed to the gene space, where they showed a highly nonuniform distribution. In both heterozygotes, more than half of the junctions fell in the std gene, making it a clear recombination hotspot in the region. However, the genetic size of std was 2-fold lower when flanked by a hemizygous 25-kb retrotransposon cluster, (ⅱ) No junctions fell in the hyproi gene in either heterozygote, making it a genie recombination coldspot. (ⅲ) No recombination occurred within the gene fragments borne on Helitrons nor within retrotransposons, so neither insertion class contributes to the interval's genetic length, (ⅳ) Unexpectedly, several junctions fell in an intergenic region not shared by all 3 haplotypes. (ⅴ) In general, the ability of a sequence to recombine correlated inversely with its methylation status. Our results show that haplotypic structural variability strongly affects the frequency and distribution of recombination events in maize.