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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution
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Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution

机译：5.5-A分辨率的真核翻译80S核糖体的低温EM结构和rRNA模型

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摘要

Protein biosynthesis, the translation of the genetic code into poly-peptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Trtticum aestivum) 80S ribosome at 5.5-A resolution. This map, together with a 6.1-A map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ～98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.
机译：蛋白质生物合成（遗传密码翻译成多肽）发生在称为核糖体的核糖核蛋白颗粒上。尽管细菌核糖体的X射线结构可用，但缺乏真核80S核糖体的高分辨率结构。使用冷冻电子显微镜和单粒子重建，我们确定了5.5-A分辨率的翻译植物（Ttticum aestivum）80S核糖体的结构。该图与酿酒酵母80S核糖体的6.1-A图一起使我们能够建模约98％的rRNA。 rRNA扩展段（ES）和可变区的准确分配揭示了独特的ES-ES和r-蛋白质-ES相互作用，从而提供了对真核核糖体的结构和进化的了解。

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  • 作者

    Jean-Paul Armache; Alexander Jarasch; Andreas M. Anger; Elizabeth Villa; Thomas Becker; Shashi Bhushan; Fabrice Jossine; Michael Habeck; Guelcin Dindar; Sibylle Franckenberg; Viter Marquez; Thorsten Mielke; Michael Thomm; Otto Berninghausen; Birgitta Beatrix; Johannes Soeding; Eric Westhof; Daniel N. Wilson; Roland Beckmann;

  • 作者单位

    Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, Ludwig-Maximilians-Universitaet Muenchen, Feodor-Lynen-Strasse 25, 81377 Munich, Germany;

    Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, Ludwig-Maximilians-Universitaet Muenchen, Feodor-Lynen-Strasse 25, 81377 Munich, Germany;

    Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, Ludwig-Maximilians-Universitaet Muenchen, Feodor-Lynen-Strasse 25, 81377 Munich, Germany;

    Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany;

    Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, Ludwig-Maximilians-Universitaet Muenchen, Feodor-Lynen-Strasse 25, 81377 Munich, Germany;

    Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, Ludwig-Maximilians-Universitaet Muenchen, Feodor-Lynen-Strasse 25, 81377 Munich, Germany;

    University de Strasbourg, Institut de Biologie Moleculaire et Cellulaire du Centre National de la Recherche Scientifique, 15 Rue Rene Descartes, 67084 Strasbourg, France;

    Department of Empirical Inference, Max Planck Institute for Biological Cybernetics, Spemannstrasse 38, 72076 Tuebingen, Germany,Department of Protein Evolution, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tuebingen, Germany;

    Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, Ludwig-Maximilians-Universitaet Muenchen, Feodor-Lynen-Strasse 25, 81377 Munich, Germany;

    Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, Ludwig-Maximilians-Universitaet Muenchen, Feodor-Lynen-Strasse 25, 81377 Munich, Germany;

    Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, Ludwig-Maximilians-Universitaet Muenchen, Feodor-Lynen-Strasse 25, 81377 Munich, Germany;

    UltraStrukturNetzwerk, Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany,Institut fuer Medizinische Physik und Biophysik, Charite, Ziegelstrasse 5-8, 10117 Berlin, Germany;

    Universitaet Regensburg, Lehrstuhl fuer Mikrobiologie, Universitaetstrasse 31, 93053 Regensburg, Germany;

    Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, Ludwig-Maximilians-Universitaet Muenchen, Feodor-Lynen-Strasse 25, 81377 Munich, Germany;

    Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, Ludwig-Maximilians-Universitaet Muenchen, Feodor-Lynen-Strasse 25, 81377 Munich, Germany;

    Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, Ludwig-Maximilians-Universitaet Muenchen, Feodor-Lynen-Strasse 25, 81377 Munich, Germany;

    University de Strasbourg, Institut de Biologie Moleculaire et Cellulaire du Centre National de la Recherche Scientifique, 15 Rue Rene Descartes, 67084 Strasbourg, France;

    Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, Ludwig-Maximilians-Universitaet Muenchen, Feodor-Lynen-Strasse 25, 81377 Munich, Germany;

    Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, Ludwig-Maximilians-Universitaet Muenchen, Feodor-Lynen-Strasse 25, 81377 Munich, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    modeling; molecular dynamics; flexible fitting;

    机译：造型;分子动力学灵活的配件;
  • 入库时间 2022-08-18 00:41:29

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