...
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >RNAi screening reveals requirement for host cell secretory pathway in infection by diverse families of negative-strand RNA viruses
【24h】

RNAi screening reveals requirement for host cell secretory pathway in infection by diverse families of negative-strand RNA viruses

机译:RNAi筛选揭示了各种负链RNA病毒感染宿主细胞分泌途径的需求

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       

摘要

Negative-strand (NS) RNA viruses comprise many pathogens that cause serious diseases in humans and animals. Despite their clinical importance, little is known about the host factors required for their infection. Using vesicular stomatitis virus (VSV), a prototypic NS RNA virus in the family Rhabdoviridae, we conducted a human genome-wide siRNA screen and identified 72 host genes required for viral infection. Many of these identified genes were also required for infection by two other NS RNA viruses, the lymphocytic choriome-ningitis virus of the Arenaviridae family and human parainfluenza virus type 3 of the Paramyxoviridae family. Genes affecting different stages of VSV infection, such as entry/uncoating, gene expression, and assembly/release, were identified. Depletion of the proteins of the coatomer complex I or its upstream effectors ARF1 or GBF1 led to detection of reduced levels of VSV RNA. Coatomer complex I was also required for infection of lymphocytic choriomeningitis virus and human parainfluenza virus type 3. These results highlight the evolu-tionarily conserved requirements for gene expression of diverse families of NS RNA viruses and demonstrate the involvement of host cell secretory pathway in the process.
机译:负链(NS)RNA病毒包含许多可导致人和动物严重疾病的病原体。尽管它们具有临床重要性,但对其感染所需的宿主因子知之甚少。我们使用水疱性口炎病毒(VSV),Rhabdoviridae家族的原型NS RNA病毒,进行了人类全基因组siRNA筛选,并确定了病毒感染所需的72个宿主基因。许多其他已鉴定的基因也需要被另外两种NS RNA病毒感染,这两种病毒是Arenaviridae家族的淋巴细胞性绒毛膜-神经炎病毒和Paramyxoviridae家族的人类3型副流感病毒。鉴定了影响VSV感染不同阶段的基因,例如进入/脱壳,基因表达和组装/释放。涂层复合体I或其上游效应子ARF1或GBF1的蛋白质耗竭导致检测到降低的VSV RNA水平。还需要Coatomer Complex I来感染3型淋巴细胞性脉络膜脑膜炎病毒和人类副流感病毒。这些结果突显了在进化上保守的各种NS RNA病毒家族基因表达的要求,并证明了宿主细胞分泌途径在此过程中的参与。

著录项

  • 来源
  • 作者单位

    School of Veterinary Medicine and Biomedical Sciences , University of Nebraska, Lincoln, NE 68583,Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583;

    School of Veterinary Medicine and Biomedical Sciences , University of Nebraska, Lincoln, NE 68583,Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583;

    School of Veterinary Medicine and Biomedical Sciences , University of Nebraska, Lincoln, NE 68583,Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583;

    School of Veterinary Medicine and Biomedical Sciences , University of Nebraska, Lincoln, NE 68583,Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583;

    National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892;

    Duke RNAi Screening Facility, Duke University Medical Center,Durham, NC 27710;

    Duke RNAi Screening Facility, Duke University Medical Center,Durham, NC 27710;

    Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583;

    University of Nebraska Medical Center, Omaha, NE 68198;

    Department of Statistics, University of Nebraska, Lincoln, NE 68588;

    School of Veterinary Medicine and Biomedical Sciences , University of Nebraska, Lincoln, NE 68583,Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    rna interference; transcription and replication; host cell factors for virus infection;

    机译:RNA干扰转录和复制;病毒感染的宿主细胞因子;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号