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Inducing sexual reproduction in a penicillin-producing fungus

机译:在产生青霉素的真菌中诱导有性生殖

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Filamentous fungi are used to produce a variety of pharmaceutical products, including statins and antibiotics, and many species have long been considered to lack a sexual cycle. Julia Bohm et al. (pp. 1476-1481) induced sexual reproduction in the filamentous, penicillin-producing fungus Peni-cillium chrysogenum, which has been considered asexual for more than a century. Performing crosses between different wild type and industrial P. chrysogenum strains on oatmeal agar medium supplemented with the compound biotin gave rise to sexual reproductive structures with viable progeny. When the authors examined the progeny they found evidence of sexual recombination at the phe-notypic and molecular levels. The authors used sexual crosses to combine previously separate traits of interest and created a P. chrysogenum strain with a high penicillin titer that lacked a contaminant called chrysogenin.
机译:丝状真菌用于生产各种药物,包括他汀类药物和抗生素,长期以来,许多物种都被认为缺乏性周期。朱莉娅·鲍姆(Julia Bohm)等。 (pp。1476-1481)诱导了青霉素丝状真菌Peni-cillium chrysogenum的有性生殖,该病已经有一百多年的历史了。在补充了复合生物素的燕麦琼脂培养基上,在不同的野生型和工业产黄单胞菌菌株之间进行杂交,产生了具有繁殖力的有性生殖结构。当作者检查后代时,他们发现了在phe-notypic和分子水平上有性重组的证据。这组作者使用性杂交方法将以前分离的感兴趣特征组合在一起,并创建了一种具有高青霉素滴度的绿脓杆菌菌株,这种青霉素滴度缺乏一种叫做黄体生成素的污染物。

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