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Identification and characterization of differentially expressed cDNAs of the vector mosquito, Anopheles Gambiae

机译:蚊子冈比亚按蚊差异表达cDNA的鉴定与鉴定

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摘要

The isolation and study of Anopheles gambiae genes that are differentially expressed in development, notably in tissues associated with the maturation and transmission of the malaria parasite, is important for the elucidation of basic molecular mechanisms underlying vector-parasite interac- tions. We have used the differential display technique to screen for mRNAs specifically expressed in adult males, females, and midgut tissues of blood-fed and unfed females. We also screened for mRNAs specifically induced upon bac- terial infection of larval stage mosquitoes.
机译:分离和研究在发育中差异表达的冈比亚按蚊基因,特别是在与疟原虫成熟和传播相关的组织中,对阐明载体-寄生虫相互作用的基本分子机制非常重要。我们已经使用差异显示技术来筛选在成年雄性,雌性以及有血和无血雌性的中肠组织中特异性表达的mRNA。我们还筛选了细菌感染幼虫期蚊子后特异性诱导的mRNA。

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