Viral RNA trafficking is inhibited in replicase-mediated resistant transgenic tobacco plants

摘要

Transgenic tobacco (Nicotiana tabacum cv. Turkish Samsun NN) plants expressing a truncated replicase gene sequence from RNA-2 of strain Fny of cucumber mosaic virus (CMV) are resistant to systemic CMV disease. This is due to suppression of virus replication and cell-to-cell move- ment in the inoculated leaves of these plants. In this study, microinjection protocols were used to directly examine cell- to-cell trafficking of CMV viral RNA in these resistant plants.