Transgenic male-sterile plant induced by an unedited atp9 gene is restored to fertility by inhibiting its expression with antisense RNA

摘要

We have previously shown that the expres- sion of an unedited atp9 chimeric gene correlated with male- sterile phenotype in transgenic tobacco plant. To study the relationship between the expression of chimeric gene and the male-sterile trait, hemizygous and homozygous transgenic tobacco lines expressing the antisense atp9 RNA were con- structed. The antisense producing plants were crossed with a homozygous male-sterile line, and the F1 progeny was analyzed.