Genetic characterization of a mammalian protein-protein interaction domain by using a yeast reverse two-hybrid system

摘要

Many biological processes rely upon protein- protein interactions. Hence, detailed analysis of these inter- actions is critical for their understanding. Due to the com- plexities involved, genetic approaches are often needed. In yeast and phage, genetic characterizations of protein com- plexes are possible. However, in multicellular organisms, such characterizations are limited by the lack of powerful selection systems.