Initiation of (-) strand DNA synthesis from tRNA_3~Lys on lentiviral RNAs: Implications of specific HIV-1 RNA-tRNA_3~Lys interactions inhibiting primer utilization by retroviral reverse transcriptases

摘要

Initiation of minus (-) strand DNA synthesis as examined on templates containing R, U5, and primer- binding site regions of the human immunodeficiency virus type 1 (HIV-1), feline immunodeficiency virus (FIV), and equine infectious anemia virus (EIAV) genomic RNA. DNA synthesis was initiated from (i) an oligoribonucleotide complementary to the primer-binding sites, (ii) synthetic tRNA_3~Lys, and (iii) natural tRNA_3~Lys, by the reverse transcriptases of HIV-1, FIV, EIAV, simian immunodeficiency virus, HIV type 2 (HIV-2), Moloney murine leukemia virus, and avian myeloblastosis virus.