X-ray structure of a vanadium-containing enzyme: Chloroperoxidase from the fungus Curvularia inaequalis

摘要

The chloroperoxidase (EC 1.11.1.-) from the fungus Curvularia inaequalis belongs to a class of vanadium enzymes that oxidize halides in the presence of hydrogen peroxide to the corresponding hypohalous acids. The 2.1 A crystal structure (R = 20/100) of an azide chloroperoxidase complex reveals the geometry of the catalytic vanadium center. Azide coordinates directly to the metal center, result- ing in a structure with azide, three nonprotein oxygens, and a histidine as ligands.