Expression cloning and functional characterization of the kidney cortex high-affinity proton-coupled peptide transporter

摘要

The presence of a proton-coupled electro- genic high-affinity peptide transporter in the apical mem- brane of tubular cells has been demonstrated by microperfu- sion studies and by use of brush border membrane vesicles. The transporter mediates tubular uptake of filtered di- and tripeptides and aminocephalosporin antibiotics. We have used expression cloning in Xenopus laevis oocytes for identification and characterization of the renal high-affinity peptide trans- porter.