首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A class of single-stranded telomeric DNA-binding proteins required for Rap1p localization in yeast nuclei.
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A class of single-stranded telomeric DNA-binding proteins required for Rap1p localization in yeast nuclei.

机译:Rap1p在酵母核中定位所需的一类单链端粒DNA结合蛋白。

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We have identified a class of proteins that bind single-stranded telomeric DNA and are required for the nuclear organization of telomeres and/or telomere-associated proteins. Rlf6p was identified by its sequence similarity to Gbp1p, a single-stranded telomeric DNA-binding protein from Chlamydomonas reinhardtii. Rlf6p and Gbp1p bind yeast single-stranded G-strand telomeric DNA. Both proteins include at least two RNA recognition motifs, which are found in many proteins that interact with single-stranded nucleic acids. Disruption of RLF6 alters the distribution of repressor/activator protein 1 (Rap1p), a telomere-associated protein. In wild-type yeast cells, Rap1p localizes to a small number of perinuclear spots, while in rlf6 cells Rap1p appears diffuse and nuclear. Interestingly, telomere position effect and telomere length control, which require RAP1, are unaffected by rlf6 mutations, demonstrating that Rap1p localization can be uncoupled from other Rap1p-dependent telomere functions. In addition, expression of Chlamydomonas GBP1 restores perinuclear, punctate Rap1p localization in rlf6 mutant cells. The functional complementation of a fungal gene by an algal gene suggests that Rlf6p and Gbp1p are members of a conserved class of single-stranded telomeric DNA-binding proteins that influence nuclear organization. Furthermore, it demonstrates that, despite their unusual codon bias, C. reinhardtii genes can be efficiently translated in Saccharomyces cerevisiae cells.
机译:我们已经确定了一类结合单链端粒DNA的蛋白质,并且是端粒和/或端粒相关蛋白的核组织所必需的。 Rlf6p通过与Gbp1p的序列相似性进行鉴定,Gbp1p是来自莱茵衣藻的单链端粒DNA结合蛋白。 Rlf6p和Gbp1p结合酵母单链G链端粒DNA。两种蛋白质均包含至少两个RNA识别基序,这是在许多与单链核酸相互作用的蛋白质中发现的。 RLF6的破坏改变了阻遏物/激活蛋白1(Rap1p)的分布,端粒相关蛋白。在野生型酵母细胞中,Rap1p定位于少量的核周斑点,而在rlf6细胞中,Rap1p则显示为弥漫性和核性。有趣的是,需要RAP1的端粒位置效应和端粒长度控制不受rlf6突变的影响,表明Rap1p的定位可以与其他Rap1p依赖的端粒功能脱钩。此外,衣藻GBP1的表达恢复rlf6突变细胞中的核周,点状Rap1p定位。藻类基因对真菌基因的功能互补表明,Rlf6p和Gbp1p是影响核组织的保守类单链端粒DNA结合蛋白的成员。此外,它表明,尽管它们具有不寻常的密码子偏向性,但仍可以在酿酒酵母细胞中有效地翻译莱茵衣藻基因。

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