首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Rep protein of tomato yellow leaf curl geminivirus has an ATPase activity required for viral DNA replication.
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Rep protein of tomato yellow leaf curl geminivirus has an ATPase activity required for viral DNA replication.

机译:番茄黄叶卷曲双生病毒的Rep蛋白具有病毒DNA复制所需的ATPase活性。

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摘要

The Rep protein of geminiviruses is the sole viral protein required for their DNA replication. The amino acid sequence of Rep protein contains an NTP binding consensus motif (P-loop). Here we show that purified Rep protein of tomato yellow leaf curl virus expressed in Escherichia coli exhibits an ATPase activity in vitro. Amino acid exchanges in the P-loop sequence of Rep causes a substantial decrease or loss of the ATPase activity. In vivo, mutant viruses carrying these Rep mutations do not replicate in plant cells. These results show that ATP binding by the Rep protein of geminiviruses is required for its function in viral DNA replication.
机译:双生病毒的Rep蛋白是其DNA复制所需的唯一病毒蛋白。 Rep蛋白的氨基酸序列包含NTP结合共有基序(P-环)。在这里,我们显示了在大肠杆菌中表达的番茄黄叶卷曲病毒的纯化Rep蛋白在体外表现出ATPase活性。 Rep的P环序列中的氨基酸交换会导致ATPase活性大大降低或丧失。在体内,携带这些Rep突变的突变病毒不会在植物细胞中复制。这些结果表明,双生病毒的Rep蛋白与ATP的结合是其在病毒DNA复制中的功能所必需的。

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