The secondary structure of type I collagen N-telopeptide as demonstrated by Fourier transform IR spectroscopy and molecular modeling

摘要

The amino-telopeptides of type I collagen have been implicated to have a crucial role in the events of fibril formation. The sequences have been highly conserved in a variety of species. Several proposals have been presented to correlate the sequence with structure and role in fibri1 formation, but no definite information on telopeptide structure has been deduced. The infrared spectrum of the amide I band, due almost entirely to the C=O stretch vibration of peptide carbonyls, has been a most useful probe for determining the secondary structures of proteins in solution. In the present study, the secondary structure of a synthetic rat alpha 1 (I) amino-telopeptide has been investigated in aqueous solution by Fourier transform infrared spectroscopy (FTIRS) using a 9-pass internal reflectance ZnSe prism cell. Conformational changes were monitored as the aqueous solution was heated from 4 to 50 deg.C by observing changes in the freauency position. To support these experimental data the telopeptide region was modeled using BIOSYM/MSI software on a Silicon Graphics R-4000, X/Z graphics workstation. The proposed telopeptide structure was energy minimized using DISCOVER CVFF repetitive build and minimize process to reduce steric hindrance and maximize H- bonding. The potential energy surface was quite low and the conformation was stabilized by only 3 H-bonds. This model suggests a telopeptidc structure that can be induced to assume a conformation favorable for binding during its interchain interaction at the collagen helix cross-link (N-telopeptide) receptor domain around collagen