環状DNAとタンパク質を用いたナノ構造体の作製:　DNA複合構造体作製の基本原理の提案と検証

摘要

ナノテクノロジーを応用した加工手法として，原子·分子を積rnみ上げてデバイスを構築させるボトムアップ的手法が注目されrnている．その手法の1つに，生体高分子であるDNAが有するrn自己組織化能に着目し，DNAを基材とした分子デバイスを構築rnしようとする研究が近年盛んに進められている．rnDNAに着目する理由としては，rn（1）DNA自体が直径約2nmのナノスケールサイズの構造体rn（2）塩基の相補性を利用した精密な連結が可能rn（3）プロトコルが確立されており安価で入手可能rn（4）自己複製機能を有し大量コピーが可能rnという点が挙げられ，DNAは遺伝物質のみならずナノ構造体をrn作製するパーツとしても大変魅力的な分子である．%Molecular self-assembling is one of the important technologies for fabrication of nano-mechanisms in the bottom-up techniques. Among many kinds of biomolecular, DNA has several advantages such as creativity of arbitrary base sequence by DNA synthesizer, sub-nm self-positioning resolution in hybridization, and well-developed cut-and-paste technique, which is attractive for nano-mechanism fabrication. This report describes the design and fabrication of DNA-based nanostructures ("DNA glasses", "DNA serial rings", and "DNA chains") using DNA ring-protein complex. An experiment has been performed to form the DNA ring using two types of DNA ("vector DNA") obtained by cutting plasmid with restriction enzyme and biotin conjugated DNA ("insert DNA"). After ligation and introduction of streptavidin modified gold nanoparticle, DNA-based nanostructures have been obtained. And then, DNA-based nanostructures were observed by an Atomic Force Microscope (AFM). By a function of the AFM micrograph processing software, a cross sectional analysis was performed. As a result, the diameter of the DNA rings was from 21 to 24 nm, which was almost the same as the theoretical value (25 nm). Hence DNA-based nanostructures were confirmed to be fabricated in the designed construction.