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Bovine Serum Albumin Immobilized-Polyvinyl Alcohol Membranes: A Study Based on Sorption, Dye Release and Protein Adsorption

机译:牛血清白蛋白固定化聚乙烯醇膜:基于吸附,染料释放和蛋白质吸附的研究

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Bovine serum albumin (BSA) immobilized polyvinyl alcohol (PVA) asymmetric membranes were prepared under glutaraldehyde concentration gradient. Morphology of the prepared membranes was analyzed by using Field Emission Scanning Electron Microscopy (FESEM) and Atomic Force Microscopy (AFM). The surface morphological studies revealed that the PVA-BSA samples resemble asymmetric membrane structure. The cross-link density of the membranes was found to influence the water sorption and dye release. In situ protein adsorption studies were performed in order to check the possibilities of this sample for biomedical applications. The results indicate that protein adsorption decreases with increase in BSA content in the membrane.View full textDownload full textKeywordsBovine serum albumin, Dye release, Polyvinyl alcohol, Protein adsorption, Protein immobilized membranes, Water sorptionRelated var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/03602559.2012.702254
机译:戊二醛浓度梯度制备牛血清白蛋白(BSA)固定化聚乙烯醇(PVA)不对称膜。通过使用场发射扫描电子显微镜(FESEM)和原子力显微镜(AFM)来分析所制备的膜的形态。表面形态研究表明,PVA-BSA样品类似于不对称膜结构。发现膜的交联密度影响水吸收和染料释放。为了检查该样品在生物医学应用中的可能性,进行了原位蛋白吸附研究。结果表明,蛋白质吸附随膜中BSA含量的增加而降低。查看全文下载全文关键词牛血清白蛋白,染料释放,聚乙烯醇,蛋白质吸附,蛋白质固定膜,水吸附相关的var addthis_config = {ui_cobrand:“ Taylor&Francis Online ”,services_compact:“ citeulike,网络振动,微博,technorati,美味,linkedin,facebook,stumbleupon,digg,google,更多”,发布号:“ ra-4dff56cd6bb1830b”};添加到候选列表链接永久链接http://dx.doi.org/10.1080/03602559.2012.702254

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