温度応答性高分子ブラシの精密設計とバイオセパレーションへの応用

摘要

現在の医療ではバイオ医薬品を用いた治療や細胞を生体に移植する再生医療が効果的な治療方法として用いられている.これらの治療法に使用するタンパク質，細胞の重要性が高まってきており，活性を損なうことなく効率的に分離•精製する機能的な分離技術の開発が求められている.そこで筆者らは，外部温度変化のみで分離対象のタンパク質，細胞との相互作用を制御して分離を行う温度応答性高分子ブラシ修飾表面の開発を行つている.本報ではこれらの温度応答性高分子ブラシの特性とバイォセパレーションへの応用について概説する.%Protein drugs and therapeutic cells are among promising current and future therapies for patients. The importance of these proteins and cells has increased in the clinical field, and protein and cell purification methods, without losing their potency or biological activity, are urgently needed. We prepared thermoresponsive polymer brushes as a new protein and cell separation tool because interactions between the polymer brushes and targeted proteins and cells can be modulated by changing the external solution temperature without using other agents that might contaminate these biologics or disrupt their function. Using these new thermoresponsive polymer brushes, a variety of different bio-separations have been performed by simple temperature changes in aqueous solutions. The present review describes the recent achievement in bioseparation using thermoresponsive polymer brushes.