首页> 外文期刊>Acta Societatis Botanicorum Poloniae >Morphogenic processes in callus tissue cultures and de novo regeneration of plants in Actinidia chinensis Planch
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Morphogenic processes in callus tissue cultures and de novo regeneration of plants in Actinidia chinensis Planch

机译:中华猕猴桃愈伤组织培养的形态发生过程和植物的从头再生

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摘要

Our experiments have confirmed the considerable disposition of leaf explants of Actinidia chinensis Planch. For induction and intensive proliferation of callus cultures, as well as, a possibility to regulate morhogenesis in vitro conditions. Under speciic culture conditions the morphogenic potential of callus cells of Actinidia chinensis was manifested both in organogenesis and somatic embryogenesis. Organogenesis was represented by induction of adventitious buds and regeneration shoots on the modi- fied Ms culture medium (Murashige and Skoog 1962) with BAP in combination with GA_3(each 1.0 mg. L~-1). Rooting of shoots was successful in modified MS medium containing IBA(0.5-1.0 mg.l~-1).
机译:我们的实验已经证实了猕猴桃叶片外植体的大量配置。对于愈伤组织培养物的诱导和密集增殖,以及在体外条件下调节致瘤性的可能性。在特定的培养条件下,中华猕猴桃愈伤组织细胞的形态发生潜能在器官发生和体细胞胚发生中都有表现。器官发生的表现是在改良的Ms培养基(Murashige和Skoog 1962)上用BAP结合GA_3(1.0 mg。L〜-1)诱导不定芽和再生芽。在含有IBA(0.5-1.0 mg.l〜-1)的改良MS培养基中,生根成功。

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