Organogenesis during plant regeneration in cotyledon culture of trifolium michelianum Savi.

摘要

Shoots of Trifolium michelianum Savi. Could be induced directly from the cotyledonary petiole or through petiole-derived callus on EC6 medium (Mahewswaran and Williams 1984) supplemented with BAP at 4.4, 8.8 and 22. ΜM. The mode of caulogenesis highly depended on cytokinin concentration in the medium. Most of the shoots regeneratedin to mature plants when transplanted to half-strong MS (Murashige and Skoog 1962). The time of caulogenic determination was set for the first day of culture.