Selection of valine - Resistance in callus culture of arabidopsis thaliana (L.) heynh. Derived from leaf explants

摘要

The selection of valine-resistant mutants was carried out in leaf explant cultsures of three Arabidopsis thaliana 9L.) Heynh. Ecotypes: C-24, RLD and Columbia. The valine concentration used for in vitro selection, lethal for seed-growing plants, has not affected callus formation and growth. However, strong inhibition of shoot regeneration ability of calli growing under Selection pressure was noticed. In total, 1043 explants were cultured on valine medium and 18 shoots were regenerated with An average frequency of 1.7 shoots per 100 calli.