Species delimitation in the Ponto-Caucasian Viola sieheana complex, based on evidence from allozymes, morphology, ploidy levels, and crossing experiments

摘要

Information from allozymes, ploidy levels, morphology, cross-compatibility and biogeography suggests that the easternmost occurrences of the Ponto-Caucasian Viola sieheana should be considered to belong to a separate species, V. caspia. Morphological characters delimitating V. sieheana and V. caspia are presented and discussed. The main distribution of V. sieheana s. str. is in the eastern Balkans, Turkey, Cyprus, and The Lebanon, and of V. caspia in the Caspio-Caucasian region westwards to Crimea and north-eastern Turkey. Their distributions in the intermediate areas remain unknown. Plants of V. sieheana s. str. from Cyprus, Greece, and Turkey were all blue-flowered and had the chromosome number 2n = 12x = 60. Its presence in the Peloponnese (Parnon, Taÿgetos, Menalon) and in the Pindhos mountains, Greece, was confirmed. Reports of plants with 2n = 8x = 40 from these stations seem to refer to the sterile hybrid V. reichenbachiana × sieheana which is locally abundant. V. caspia from Azerbaijan had 2n = 8x = 40 and had either whitish or blue flowers. In spite of sympatry and almost complete cross-compatibility, the two corolla colour morphs showed strong allozymic differentiation in four out of seven isozyme systems, suggesting reproductive isolation and incipient speciation. Within section Viola, V. caspia and V. sieheana seem no more closely related to each other than to either of the other investigated European species. The possibility that V. sieheana is an ancient allopolyploid involving the Greek narrow endemic V. dirphya (2n = 40) is discussed.