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Modulation of plumbagin production in Plumbago zeylanica using a single-chain variable fragment antibody against plumbagin

机译:使用针对李白蛋白的单链可变片段抗体调节玉米花中李白蛋白的生产

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摘要

A single-chain variable fragment antibody (scFv) against plumbagin (PL) accumulated the PL production in the hairy roots of Plumbago zeylanica. Recombinant Agrobacterium rhizogenes (ATCC 15834) containing an scFv gene against PL (PL-scFv) were obtained through triparental mating and transformed into P. zeylanica to induce PL-scFv protein in the hairy roots. Up to 40 μg recombinant PL-scFv were expressed per milligram of soluble protein in transgenic P. zeylanica hairy root cultures. The mean PL content obtained from transgenic hairy roots (12.24 μg/100 mg dry weight) exhibited 2.2 times higher than those obtained from wild-type (5.48 μg/100 mg dry weight). The high correlation between the PL-scFv expression level and PL content of the recombinant plants suggested that the PL biosynthesis pathway had been modulated by the expression of PL-scFv protein in the hairy roots of P. zeylanica.
机译:单抗变蛋白单抗(PLF)的单链可变片段抗体(scFv)在玉米花的毛状根中积累了PL的产生。通过三亲交配获得了含有抗PL的scFv基因的重组发根农杆菌(ATCC 15834),并将其转化到zeylanica毛状根中诱导PL-scFv蛋白。每毫克可溶蛋白在转基因zeylanica毛状根培养物中表达多达40μg重组PL-scFv。从转基因毛根获得的平均PL含量(12.24μg/ 100 mg干重)显示出比从野生型(5.48μg/ 100 mg干重)获得的平均PL含量高2.2倍。重组植物的PL-scFv表达水平和PL含量之间的高度相关性表明,PL-scFv蛋白在玉米。毛根中的表达调节了PL的生物合成途径。

著录项

  • 来源
    《Plant Cell Reports》 |2012年第1期|p.103-110|共8页
  • 作者单位

    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan;

    Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand;

    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan;

    Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, 85 Warinchamrap, Ubon Ratchathani, 34190, Thailand;

    Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, 859-3298, Japan;

    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan;

    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Agrobacterium rhizogenes; Hairy root; Plumbagin; Plumbago zeylanica; Single-chain variable fragment antibody;

    机译:发根农杆菌;毛发根;铅蛋白;西兰花;单链可变片段抗体;
  • 入库时间 2022-08-18 01:45:39

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