Simulations of space-clamp errors in estimating parameters of voltage-gated conductances localized at different electrotonic distances

摘要

The ability to correct parameters of voltage-gated conductances measured under poor spatial control by a point voltage clamp could rescue much flawed experimental data, but requires a detailed understanding of errors caused by poor space clamp. We evaluated errors in simulated voltage-clamp experiments on a soma with a single (0.5 λ) cylindrical process having a patch of voltage-dependent Hodgkin-Huxley channels placed at various distances from the soma. Kinetic and steady-state parameters were obtained by fitting the current curves. Most parameter errors increased as the patch was located more distally on the neurite.