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首页> 外文期刊>Molecular Breeding >Molecular characterization of latent fertility restorer loci for Honglian cytoplasmic male sterility in Oryza species
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Molecular characterization of latent fertility restorer loci for Honglian cytoplasmic male sterility in Oryza species

机译:水稻红连胞质雄性不育潜伏恢复基因位点的分子特征。

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摘要

In Oryza species, several distinct cytoplasmic male sterility (CMS) systems have been identified. Honglian (HL) is one of the mainstream breeding CMS lines in large-scale commercial production in China and southern Asia. In order to increase restorer diversity, we screened 130 Oryza accessions to find new restorer loci for HL-CMS. We analyzed the restoring ability and the diversity of molecular markers cosegregated with Rf5 (Milyang23) and Rf6(t) (9311) for HL-CMS. Molecular and genetic analyses revealed that eight accessions, including one cultivar and seven wild rice, showed polymerase chain reaction (PCR) patterns different from Rf5 and Rf6(t), and each of them harbored a pair of Rf genes unallelic to Rf5 and Rf6(t) for HL-CMS. Furthermore, Northern blot revealed that the Rf loci in the eight accessions showed three types of processing manners of HL-CMS-related transcripts, including endonucleolytic cleavage, partial endonucleolytic cleavage, and degradation of dicistronic atp6-orfH79 messenger RNA (mRNA). The Rf genes for HL-CMS in AA-genome Oryza species elucidated by genetic and molecular analyses will be highly beneficial to sustainable development of hybrid rice.
机译:在稻属物种中,已经鉴定出几种不同的细胞质雄性不育(CMS)系统。红莲(HL)是中国和南亚大规模商业生产中的主流育种CMS系列之一。为了增加恢复子的多样性,我们筛选了130种Oryza入藏物,以寻找用于HL-CMS的新恢复子基因座。我们分析了与Rf5(Milyang23)和Rf6(t)(9311)共同分离的HL-CMS的恢复能力和分子标记的多样性。分子和遗传分析显示,包括1个品种和7个野生稻在内的8个种质表现出与Rf5和Rf6(t)不同的聚合酶链反应(PCR)模式,并且每个都有一个与Rf5和Rf6等位的等位基因。 t)用于HL-CMS。进一步的Northern印迹分析显示,这8个种质中的Rf基因座显示了与HL-CMS相关转录本的三种加工方式,包括内切核酸酶切,部分内切核酸酶切和双顺反子atp6-orfH79信使RNA(mRNA)的降解。通过遗传和分子分析阐明的AA基因组稻米中HL-CMS的Rf基因将对杂交水稻的可持续发展非常有利。

著录项

  • 来源
    《Molecular Breeding》 |2012年第4期|p.1699-1706|共8页
  • 作者单位

    Key Laboratory for Biotechnology of the National Committee for Nationalities, College of Life Sciences, South-Central University for Nationalities, Wuhan, 430074, China;

    State Key Laboratory for Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China;

    State Key Laboratory for Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China;

    State Key Laboratory for Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China;

    State Key Laboratory for Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Cytoplasmic male sterility; Fertility restorer; Molecular marker; Oryza species;

    机译:细胞质雄性不育;生殖力恢复;分子标记;Oryza;

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