Ultra-thin poly(dimethylsiloxane) film-coated glass capillaries for fluoroimmunosensing applications

摘要

Methods for homogeneous coverage of surfaces with biomolecules are critical for the development of bio-analytical microsystems. Here we present a simple method for coating glass capillaries with an ultra-thin poly(dimethylsiloxane) (PDMS) film in order to be used as optical waveguide fluoroimmunosensors. A model rabbit γ-globulin binding assay was employed for the evaluation of modified capillaries in terms of protein binding capacity and protein coating homogeneity. It was found that the PDMS-modified capillaries provided excellent protein coating homogeneity inside each one capillary (CVs 2.9-6.6%) and between-capillaries repeatability (CVs 2.1-5.0%) compared to aminosilanized ones. Furthermore, the waveguiding properties of the glass were not affected by the proposed surface modification.