Temperature and catecholamine effects on metabolism of perfused isolated rabbit muscle

摘要

Biceps brachii muscle was isolated from anaesthetized New Zealand rabbits and perfused for 30 min with bovine red cells, while electrically stimulated at 0.1 Hz, 50 V. Temperature was maintained at 34 (physiological temperature of this muscle, 36 or 38 deg. C in the first experiment (Exp 1). In the second experiment (Exp 2), temperature was maintained at either 34 or 38 deg. C and the muscle was perfused with 2 ng/ml adrenaline and 10 ng/ml noradrenaline for 5 min during the perfusion. Whatever the temperature, twitch force decreased (P＜0.01) during perfusion indicating muscular fatigue.