Blood-brain barrier transport of Tat peptide and polyethylene glycol decorated gelatin-siloxane nanoparticle

摘要

Contemporary treatment of brain diseases has been hampered by limited drug delivery across the blood-brain barrier (BBB). Herein, gelatin-siloxane nanoparticles (GSNPs) with controlled size and surface charge were synthesized through a two-step sol-gel process. In order to increase the efficiency of brain targeting, HIV-derived Tat peptide and PEG were further grafted onto GS NPs (Tat-PEG-GS NPs). In vivo imaging and TEM results indicated Tat-PEG-GS NPs could not only escape the capture by reticulo-endothelial system but also cross BBB and reach central nervous system of mice. Hence, Tat-PEG-GS NPs might represent a new type of non-viral vector for the delivery of drug or therapeutic DNA to brain by crossing BBB.