Synthesis of high quality dual-mode encoded phenolic resin microspheres based on Fluorescence and Surface Enhanced Raman Spectra

摘要

Graphical abstractDisplay OmittedHighlights•Phenolic resin microspheres encoded by Fluorescence and SERS spectra were fabricated.•The phenolic resin microspheres possess autofluorescence properties.•SERS-fluorescent dual model encoding greatly expanded the coding capacity.•The encoded microspheres presented promising application prospect.AbstractEncoded microsphere suspension array is promising for multiplex bioassay. Novel high quality phenolic resin (PR) microspheres encoded by Fluorescence and Surface Enhanced Raman Spectra (SERS) were fabricated in the present work. The PR microspheres possessed autofluorescence property and could be jointly encoded with other fluorescent molecules including fluorescein isothiocyanate. The fluorescent encoded PR microspheres were modified with Ag nanoparticles and Raman label molecules for combination of SERS encoding, which is rarely reported. The dual-mode encoded microspheres were evaluated by MTT assay and flow cytometry. The results showed the high quality encoded microspheres presented promising application prospect in biomarker and detection of antibody, DNA and RNA.