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Testosterone Metabolism in Imposex and Normal Ilyanassa bbsoleta: Comparison of Field and TBTA Cl-Induced Imposex

机译:Imposex和正常Ilyanassa bbsoleta中的睾丸激素代谢:田间和TBTA Cl诱导的Imposex的比较

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摘要

Tributyltin (TBT) is a marine biocide that alters activity of cytochrome P450 (P450) monooxygenases and elicits androgenization in gastropod molluscs. This study was conducted to determine whether testosterone metabolism was altered in field collected and TBT-induced imposex female mud snail, I. obsoleta. Normal and imposex snails were collected from two field sites. Imposex was induced by exposing normal snails to 2.5, 5.0, 10, 20, or 200 ng TBT l~(-1) seawater for 45 days at 19℃. Testosterone metabolism was studied by exposing snails to [~(14)C]-testosterone for 24 h and determining conversion by phase Ⅰ (P450s and reductases) and phase Ⅱ (transferases) enzymes. All snails took up similar levels of testosterone from seawater, and radioactivity partitioned primarily into gonad/digestive gland complex. Testosterone was metabolized primarily by reductases. Field collected imposex snails had decreased rates of reduced metabolites, while there was no overall change in testosterone metabolism in TBT-induced imposex snails. These findings indicate that field-collected imposex snails metabolize testosterone differently from normal snails, as well as from TBT-induced imposex snails.
机译:三丁基锡(TBT)是一种海洋生物杀伤剂,可改变细胞色素P450(P450)单加氧酶的活性并引起腹足动物软体动物雄激素化。进行了这项研究,以确定田间收集的睾丸激素代谢是否发生改变,以及TBT诱导的雌性田螺(I. obsoleta)是否受到影响。从两个野外地点收集了普通蜗牛和Imposex蜗牛。将正常蜗牛在19℃下暴露于2.5、5.0、10、20或200 ng TBT l〜(-1)海水中45天,即可诱发Imposex。通过将蜗牛暴露于[〜(14)C]-睾丸酮24小时,并通过Ⅰ期酶(P450和还原酶)和Ⅱ期酶(转移酶)来确定睾丸激素的代谢。所有蜗牛都从海水中吸收相似水平的睾丸激素,并且放射性主要划分为性腺/消化腺复合体。睾丸激素主要通过还原酶代谢。在田间采集的蜗牛,其代谢产物减少的速率降低,而TBT诱导的蜗牛体内的睾丸激素代谢没有整体变化。这些发现表明,田间采集的钉螺与正常钉螺以及TBT诱导的钉螺的代谢睾丸激素不同。

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