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Assessment of PAH Pollution in Coastal Areas from the NW Mediterranean through the Analysis of Fish Bile

机译:通过鱼类胆汁分析评估地中海西北部沿海地区的PAH污染

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Polycyclic Aromatic Hydrocarbon (PAH) pollution in coastal areas from the NW Mediterranean has been assessed by measuring bile PAH metabolites in two fish species, the red mullet - Mullus barbatus-and the sea comber - Serranus cabrilla - Bile crude samples were directly analysed by LC-fluorescence at the excitation/emission wavelengths of benzo[a]pyrene (380/430 nm). Subsequently, bile samples were hydrolysed and analysed by (a) LC-fluorescence at the excitation/emission wavelength pairs of naphthol (290/335 nm) and pyrenol (345/395 nm), and (b) gas chromatography-mass spectrometry (GC-MS) for the determination of individual PAHs. Qualitative and quantitative differences were recorded among sampling sites. Both species allowed us to detect different pollution gradients, the highest levels of FACs were observed in organisms caught in the vicinity of big industrial cities, whereas the lowest levels were recorded in organisms from Corsica and Sardinia. The obtained results highlight the usefulness of the LC-fluorescence technique as an initial screening method to assess PAH exposure in coastal fish.
机译:通过测量两种fish鱼中的PAH代谢产物来评估西北地中海沿岸地区的多环芳烃(PAH)污染,其中两种fish鱼是红let鱼-Mullus barbatus-海梳子-Serranus cabrilla-胆汁原油样品通过LC直接分析-在苯并[a] py的激发/发射波长(380/430 nm)下发出荧光。随后,胆汁样品被水解并通过(a)萘酚(290/335 nm)和苯酚(345/395 nm)的激发/发射波长对的LC荧光分析和(b)气相色谱-质谱(GC)进行分析-MS)用于确定各个PAH。记录采样点之间的定性和定量差异。两种物种都使我们能够检测到不同的污染梯度,在大型工业城市附近捕获的生物中,FAC的含量最高,而在可西嘉岛和撒丁岛的生物中,FAC的含量最低。获得的结果凸显了LC荧光技术作为评估沿海鱼类PAH暴露的初步筛选方法的有用性。

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