RNA-seq analysis reveals the significant effects of different light conditions on oil degradation by marine Chlorella vulgaris

摘要

MarineChlorella vulgaris, an efficient hydrocarbon-degrading organism, is easily affected by light. In this study, we investigated the direct effects of different light conditions on crude oil degradation byC. vulgarisand its crude enzyme. Under 12 h illumination, the crude enzyme improved hydrocarbon removal by 39.36%, whereas the addition of the enzyme andC. vulgarisincreased the degradation rate by 121.73%. Conversely, the addition of enzyme under heterotrophic condition was negatively related to oil degradation byC. vulgaris, and the degradation rate decreased from 74.32% to 48.65% and further reduced by 34.54%. The results of RNA sequencing analysis suggested that hydrocarbons removal was attributed toC. vulgarismetabolism in heterotrophic physiological state. While enhanced removal efficiency of hydrocarbons was achieved in mixotrophic physiological state due to the coupling ofC. vulgarismetabolism with photocatalytic oxidation. Functional enzymes played key roles in photocatalysis and biodegradation processes.