Monoclonal and Polyclonal Antibodies Against Fish Vitellogenin for Use in Pollution Monitoring

摘要

Two monoclonal antibodies (KB-1 and BN-5) and one polyclonal antibody (AA-1) were prepared against purified vitellogenin (Vtg) form Atlantic salmon (Salmo salar), and tested for their ability to bind Vtg from plasma of various other fish species. The Mab KB-1 was found to bind specifically to Vtg form the Salmo species, Atlantic salmon (Salmo salar) and brown tout (Salmo trutta). The Mab BN-5 showed a much wider cross-reactivity, binding to Vtg from both Salmoniformes species (Alantic salmon, brown trout, rainbow trout and Arctic Charr) and Pleuronectiformes species (turbot and halibut).