Applications of a New Method for Measuring the Total Oxyradical Scavenging Capacity in Marine Invertebrates

摘要

A new method is described for measuring the total oxyradical scavenging capa- city (TOSC) in biological tissues. The TOSC-assay is based on the reaction between peroxyl radicals and α-keto-γmethiolbutyric acid which is oxidized to ethylene. The presence within the tissues of molecules that scavenge for peroxyl radicals generated by thermal homolysis of 2,2＇ azo-bis-amidinopropane quanti- tatively reduce ethylene production. Cytosol from invertebrates showed a higher ROO° absorbance capacity than that of rat liver and in all the species endogen- ous soluble antioxidants provided greater protection than proteins.