Structural Variations of HCL-Demineralized Biotic Bones of Different Tissue Origins

摘要

Demineralized bone matrices were proved to induce bone formation when implanted in animals or human. None has dealt with bone morphology, chemical and structural variations as a result of acid treatment. In the present study demineralization by 0.6 M HCI, of cranial and femur dog bones was carried out. Consequent changes in main chemical constituents, calcium (Ca~2+), inorganic phosphorus (Pi) and total protein (Pr) were determined spectrophotometrically. Crystalline structure was assessed by XRD. Morphological changes were studied using Hg-porosimeter and SEM supplemented by EDAX. Results are discussed in relation to chemical, crystalline and morphological structure of bone matrices.