Experimental Study on Construction of Vascularized Bone Graft with Osteoinductive Calcium Phosphate Ceramics In Vivo

摘要

This study was to aimed to explore a new method for fabricating a bone substitute, which is constructed of vascularized bone graft with osteoinductive calcium phosphate ceramics in vivo. A total of 32 small and 16 big ceramic cylinders were prepared, and bone-like apatite layer was formed on the surface and the pore walls of ceramics in revised simulated body fluid (RSBF). The smaller ceramics cylinders were implanted in dorsal muscles of dogs to evaluate the osteoinductive capacity. The bigger cylinders were mixed with fresh bone tissue and were implanted near a branch of femoral blood vessel in the dog's leg muscles to construct vascularized bone grafts. After implantation for 6 and 12 weeks, the specimens were harvested. The specimens were evaluated by blood vessel staining, histological observation, tetracycline fluorescence labeling, ~(99m)Tc-MDP SPECT and mechanical tests. The results showed that bone formation was found in all the samples and bone grafts had good blood supply. This result indicated that this kind of calcium phosphate ceramics had a good osteoinductivity and that vascularized bone grafts could be constructed with osteoinductive calcium phosphate ceramics in vivo.