Osteogenic Potential of Cryopreserved/ thawed Human Bone Marrow-derived Mesenchymal Stem Cells

摘要

Cryopreserved human bone marrow-derived mesenchymal stem cells were thawed and cultured on tissue culture polystylene dishes. Viability of the cells immediately after thawing was 82%. The cells treated with dexamethasone formed abundant mineralized nodules at their extracellular regions after about culture day 14, while the cultures without dexamethasone never exhibited bone formation. The calcein uptake into the bone matrices was detected in the dexamethasone treated cells with the increase in fluorescent intensity with culture periods but not in the non-treated cells. These results suggest that human bone marrow-derived mesenchymal stem cells retain bone-forming capability even after cryopreservation/thawing.