Copper(Ⅱ)-Quenched Oligonucleotide Probes for Fluorescent DNA Sensing

摘要

Cu complexes with free coordination sites efficiently quench fluorophores with ligating properties in molecular beacon oligonucleotide probes. An advantage of this over existing probe types, which display either through space or weak contact interaction between fluorophore and quencher, is the possibility of tuning and optimizing the strength of this interaction, using for example chelating fluorophores. This should facilitate the design of superior probes with very high signal-to-noise performance in which nonspecific interactions with bioanalytical samples (leading to false positive signals) are blocked.