Three-Layer Composite Magnetic Nanoparticle Probes for DNA

摘要

Gold nanoparticles functionalized with oligonucleotides are important reagents in many highly sensitive and selective DNA and protein detection schemes.They are also key building blocks in several assembly schemes that take advantage of the chemically programmable,sequence-specific hybridization properties of nucleic acids.The ubiquitous use of gold nanoparticles in biodetection,as opposed to other compositions,stems from the existence of well-established straightforward methods for the functionalization of the gold surface with ligands containing thiol or disulfide groups,the cooperative binding properties of the probes,their intense optical properties,and their catalytic properties.Each of these properties leads to a selectivity or sensitivity advantage over conventional probes.