Investigating Bacterial N-Linked Glycosylation:Synthesis and Glycosyl Acceptor Activity of the Undecaprenyl Pyrophosphate-Linked Bacillosamine

摘要

Asparagine-linked protein glycosylation has long been considered a unique feature of the eukaryotic kingdom;however,recent studies have revealed the presence of N-linked glycoproteins in Campylo-bacter jejuni,a gram-negative bacterium that is involved in human gastroenteritis.The process of N-linked glycosylation in C.jejuni displays significant similarities to the analogous process in eukary-otes.2 A series of glycosyltransferases act sequentially to assemble a glycan donor on a polyisoprenyl carrier(undecaprenyl pyrophos-phate,Und-PP),which is transferred to the asparagine side chain of proteins at the N-X-S/T consensus sequence.