A General Strategy for the Assignment of Aliphatic Side-Chain Resonances of Uniformly ~(13)C,~(15)N-Labeled Large Proteins

摘要

For proteins smaller than 25 kDa,backbone and side-chain assignments can be obtained using uniformly ~(13)C,~(15)N-labeled proteins with triple resonance experiments.Thus,structure determination by NMR is very suitable for this type of small proteins.The same methods may fail,however,when applied to proteins larger than 30 kDa due to increased transverse relaxation rates.