Promiscuous Fatty Acyl CoA Ligases Produce Acyl-CoA and Acyl-SNAC Precursors for Polyketide Biosynthesis

摘要

The study of bioactive natural products has undergone rapid advancement with the cloning and sequencing of large numbers of gene clusters.Along with the development of expression systems,the genetic reconstitution necessitates that the heterologous hosts possess substrate pools that could be coordinately supplied for biosynthesis.Polyketide synthases (PKS) utilize acyl-coenzyme A (CoA) precursors and synthesize polyketides by repetitive decarboxylative condensations.Acyl-CoAs in the cell are synthesized through multiple metabolic routes.The biotin-dependent acyl-CoA carboxylases are the major source of dicarboxylate extender units.