Cerasome as an Infusible,Cell-Friendly,and Serum-Compatible Transfection Agent in a Viral Size

摘要

As the function of nucleic acids continues to expand,there is growing need for efficient delivery of functional nucleic acids.Since the discovery of lipofection,cationic lipids have been widely used as transfection agents in gene delivery.They form liposomal bilayers in water.Polyanionic DNAs are readily bound to the resulting cationic liposomes to give complexes (lipoplexes) which are taken in the cells via endocytosis to ultimately result in expression of the encoded gene.We are concerned about lipoplex formation.The bilayer-keeping forces are not very strong,and liposomes are not so rigid or robust.They are potentially fusible with cell membranes and hence toxic.They also easily undergo DNA-mediated/induced cross-linking/fusion to give larger particles ( 100 nm) that have lower endocytosis susceptibility and poorer vascular mobility.