Lysine-60 in Copper Chaperone Atox1 Plays an Essential Role in Adduct Formation with a Target Wilson Disease Domain

摘要

Using near-UV circular dichroism as a new tool to probe Atoxl-Wilson disease protein (WD) metal-binding domain interactions, in combination with molecular dynamics (MD) simulations, we here demonstrate that Atox1 forms a stable Cu-dependent adduct with a metal-binding domain of WD which is modulated by key residues in proximity to the metal-binding site.