Large Protein Complexes with Extreme Rotational Correlation Times Investigated in Solution by Magic-Angle-Spinning NMR Spectroscopy

摘要

Solution-state NMR spectroscopy has been very successful in the past in characterizing supramolecular protein assemblies (with molecular weights of up to 1 MDa) through the use of TROSY-type techniques.However, even at very high magnetic fields, low molecular tumbling rates limit the applicability of solution-state NMR spectroscopy in the investigation of these systems. The problem that large rotational correlation times impose on the experimental spectral quality can potentially be overcome by encapsulating a protein in a reversed micelle dissolved in a low-viscosity fluid.