Design of an Encodable Tyrosine Kinase-Inducible Domain: Detection of Tyrosine Kinase Activity by Terbium Luminescence

摘要

Tyrosine kinases are critical mediators of intracellular signaling that are misregulated in numerous cancers. Tyrosine kinase inhibitors are therefore emerging as important therapeutics in oncology. In addition, tyrosine kinase signaling is broadly implicated as important in other diseases, including diabetes, heart disease, and viral and bacterial infectivity. The importance of tyrosine phosphorylation in disease has inspired the development of numerous approaches to interrogate tyrosine kinase-mediated signaling.