High Fidelity of Base Pairing by 2-Selenothymidine in DNA

摘要

The base pairs are the contributors to the sequence-dependent recognition of nucleic acids, genetic information storage, and high fidelity of DNA polymerase replication and RNA polymerase transcription. The base hydrogen bonding and stacking also contribute to the folding and structure of DNA and RNA. To increase RNA structure and function diversities, wobble base pairs (such as U/G) are involved in RNAs, including ribozymes and viral and rRNAs. However, the wobble base pairing, where T (or U) pairs with G instead of A, reduces specific base-pairing recognition and compromises the high fidelity of the enzymatic polymerization, especially when G or T on the template is modified. The wobble base pairing can increase misrecognition and misincorporation during nucleic acid polymerization.