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Recognition-Controlled Membrane Translocation for Signal Transduction across Lipid Bilayers

机译:跨脂质双层信号传导的识别控制的膜移位。

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摘要

Membrane signaling proteins transduce information across lipid bilayer membranes in response to extra-cellular binding of chemical messengers. The design of chemical systems that initiate transmembrane signal transduction through molecular binding events is a critical step toward preparing responsive synthetic vesicles. Here we report a vesicle-based signaling system controlled by a metal cation binding event. Competition between binding of copper ions to a membrane-embedded synthetic transducer and to an extra-vesicle messenger (EDTA) is used to control translocation of the transducer across the lipid bilayer. The translocation process is coupled to activation of a catalyst that turns over encapsulated substrates on the inside of the vesicle to generate an amplified fluorescence output signal. External EDTA and copper ions can be used to reversibly switch catalysis inside the vesicles on and off in a controlled manner.
机译:膜信号蛋白响应于化学信使的细胞外结合而跨脂质双层膜转导信息。通过分子结合事件启动跨膜信号转导的化学系统的设计是制备反应性合成囊泡的关键步骤。在这里我们报告由金属阳离子结合事件控制的基于囊泡的信号系统。铜离子与嵌入膜的合成换能器与囊外信使(EDTA)结合之间的竞争用于控制换能器跨脂质双层的转运。易位过程与激活催化剂有关,该催化剂翻转囊泡内部的封装底物以产生放大的荧光输出信号。外部EDTA和铜离子可用于以受控方式可逆地打开和关闭囊泡内部的催化作用。

著录项

  • 来源
    《Journal of the American Chemical Society》 |2017年第18期|6461-6466|共6页
  • 作者单位

    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom;

    Department of Chemistry, University of Sheffield, Sheffield S3 7HF, United Kingdom;

    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-18 03:07:57

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