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Effect of dextran sulfate sodium and acute exercise on mouse intestinal inflammation and lymphocyte cytochrome c levels

机译:硫酸右旋糖酐钠和急性运动对小鼠肠道炎症和淋巴细胞细胞色素c水平的影响

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Aim. Athletes may experience gastrointestinal disturbances during intense exercise. Using a mouse model, we determined the influence of acute exercise (AE) and dextran sulfate sodium (DSS), a chemical known to induce intestinal inflammation, on: 1) inflammatory changes within small and large intestine, 2) extent of cell death as measured by cytochrome c levels in intestinal lymphocytes (IL) and 3) the effects of bovine lactoferrin (bLf), a dietary protein with anti-inflammatory properties, on these parameters. Methods. DSS was given as 5 % w/v in water for 4 days. AE consisted of 3 bouts of 90 min of exhaustive treadmill exercise, each separated by 24 h, with sacrifice before, immediately after, or 24 h after the final exercise bout. Mice were fed 2% bLf or control diet for 2 weeks before AE or DSS. Tissue inflammation was determined by histology and IL cytochrome c levels by Western blotting.rnResults. AE increased plasma 8-iso-PGF2α, a marker of oxi-dative stress, immediately after relative to before exercise (P<0.01). Cytochrome c levels were elevated following bLf (P<0.01) and DSS (P<0.05) treatment whereas AE had no significant effect. DSS, but not AE, produced histological changes suggestive of intestinal inflammation with no attenuation by bLf.rnConclusion. Three bouts of AE were not associated with intestinal inflammation or IL death in this animal model. Gastrointestinal disturbances arising from intense exercisernin humans may not be due to direct inflammatory damage although this remains to be determined clinically.
机译:目标。在剧烈运动中,运动员可能会遇到胃肠道不适。使用小鼠模型,我们确定了急性运动(AE)和硫酸葡聚糖硫酸钠(DSS)(一种已知会引起肠道炎症的化学物质)对以下方面的影响:1)小肠和大肠内的炎症变化,2)细胞死亡程度通过肠淋巴细胞(IL)中细胞色素c的水平检测3)牛乳铁蛋白(bLf)(一种具有抗炎特性的饮食蛋白)对这些参数的影响。方法。 DSS以5%w / v的量在水中给予4天。 AE包括3轮90分钟的详尽的跑步机运动,每轮间隔24 h,并在最后一次运动之前,之后或之后24 h进行牺牲。在AE或DSS之前2周,给小鼠喂食2%bLf或对照饮食。通过组织学测定组织炎症,通过Western印迹测定IL细胞色素c水平。相对于运动前,AE随即立即增加血浆8-iso-PGF2α(一种氧化应激指标)(P <0.01)。 bLf(P <0.01)和DSS(P <0.05)处理后细胞色素c水平升高,而AE没有明显作用。 DSS而不是AE产生组织学改变,提示肠道炎症,但bLf没有减弱。结论。在此动物模型中,三轮AE与肠道炎症或IL死亡无关。尽管强烈的运动损伤引起的胃肠道不适可能不是直接的炎症损害,尽管这仍需临床确定。

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