Decolorization and wood degrading ability of locally isolated basidiomycetes

摘要

The decolorization of synthetic dyes was evaluated using locally isolated basidiomycetes. Out of one hundred and twenty one samples collected, three best isolates were selected on the basis of their guaiacol oxidation and CBB G 250 decolorization potential on solid media, out of which AFC 25 has shown some distinct ability to decolorize all synthetic dyes. Synthetic dyes like anthraquinonic, azo and triphenyl methane were tested for decolourization at room temperature, pH 6 and 0.01% w/v concentration in liquid culture. Maximum decolourization observed in BPB to the extent of 97.57%. In wood weight loss assay, the percent wood weight loss was highest with AFC 25 (31.35%). The species was identified as Pleurotus nebrodensis (Inzenga) Quel by partial sequence analysis and the culture was deposited in the National Fungal Culture Collection of India (Accession No NFCCI 2060) while the sequence was submitted to Genebank (JF714975).