A Novel Biopolymer Nano-Complex Based on Fish Scale Collagen, Konjac Glucomannan, Camellia Chrysantha Polyphenols and Ginsenoside Rb1: Preparation, Characterization and Its Bioactivity

摘要

A nano-complex containing fish scale collagen, konjac glucomannan, camellia chrysantha polyphenols and ginsenoside Rb1 (GCPR) have been prepared successfully by gelation method. Infrared spectroscopy (IR), scanning field emission scanning electron microscopy (FESEM), Energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), ultraviolet-visible spectroscopy (UV-Vis) were used to determine characteristics of obtained GCPR nano-complex. The GCPR nano-complex is in spherical shape with the average particle size is 115.4 nm (95.1%) and 593.0 nm (4.1%). Polyphenols and ginsenoside Rb1 could interact with collagen and konjac glucomannan through hydrogen bonding and dipole-dipole interactions, therefore, the drug release from the GCPR nano-complex in pH 2 buffer solution (simulated gastric fluid in the human body) and pH 7.4 buffer solution (simulated intestinal fluid in the human body) could be controlled. In particular, biological activity of the GCPR nano-complex in inhibition of cancer cells, anti-inflammatory and antioxidant also was investigated. The obtained results show that the GCPR nano-complex exhibits a positive effect on inhibition of cancer cells and anti-inflammatory in comparison with polyphenol or ginsenoside Rb1. Besides, the GCPR nano-complex and polyphenol also exhibit non-toxic to normal cells and antioxidation ability. This nano-complex is promising for application in biomedicine.[GRAPHICS].